Exome Sequencing

15-24 April 2012

Wellcome Trust Genome Campus, Hinxton, Cambridge

Now closed for applications. To be kept updated on dates for the 2013 course, please email us.

Course summary

This new, laboratory-based training course provides a comprehensive overview of exome sequencing, also known as pulldown, sequence capture and targeted sequencing.
Participants will gain practical laboratory experience as well as training in downstream data analysis, interpretation and validation.

Solution hybrid capture is used to enrich for all known exon sequences (the exome) or for customised regions from genomic DNA for the purpose of sequencing the regions of interest.The enriched targets are sequenced using next generation technology to a high coverage and analysed for variants.

Course programme

Material covered will include:

• Sample quality assessment and logistics
• DNA fragmentation and quantification
• DNA library preparation
• Sequence capture
• Bait design
• Overview of Illumina sequencing
• Sequence mapping
• QC metrics
• SNP/insertion/deletion calling
• SNP consequence analysis
• Protein impact analysis
• Case/control analysis
• Rare SNP discovery
• Copy number variation

Teaching will be complemented by opportunities to interact with academic and technical experts working at the cutting edge of the field.

Course instructors

• Dr Cordelia Langford (Wellcome Trust Sanger Institute, Cambridge)
• Dr Peter Ellis (Wellcome Trust Sanger Institute, Cambridge)
• Dr Robert Andrews (Wellcome Trust Sanger Institute, Cambridge)
• Dr Matthew Breen (North Carolina State University, USA)

Guest speakers TBC shortly

How to apply

Prerequisites
Applicants should be post-doctoral scientists or 3rd Year PhD students undertaking their own research or core-lab service providers.Applicants must be familiar with standard molecular biology lab techniques. Basic UNIX skills and the ability to confidently use Excel are also required.

Cost
The course tuition fees are subsidised by the Wellcome Trust for scientists based in academic institutions anywhere in the world. This is a residential course, without exception, and there is a registration fee of £995 towards board and lodging for non-commercial applicants. The fee for commercial (non-academic) applicants is £4000.

Bursaries
Limited bursaries are available for non-commercial applicants (50 per cent of fee) and are subject to open competition. Please see our bursaries page for application information and terms and conditions.

Applications
Applicants will be required to complete an application form containing a 300-word outline of the relevance of the course to their research. Please note that documentation supporting their application will be required from the applicant's supervisor/head of department.

To submit an application, please either:

• email for an application form to advancedcourses@hinxton.wellcome.ac.uk
• download the form [Word 116KB] and our terms and conditions [Word 28KB].

Applications can be accepted by post or email. Sponsors' supporting letters must be returned, signed, by fax or post under separate cover. Please note: Incomplete applications will be rejected.

Applications and letters of support should be sent to:

Wellcome Trust Advanced Courses
Wellcome Trust Genome Campus
Hinxton
Cambridgeshire CB10 1SA
UK

F +44 (0)1223 495130
E advancedcourses@hinxton.wellcome.ac.uk