Feature: Nuts and Bolts - Mitochondria
27 March 2012. By Chrissie Giles
While you may or may not have your mum's eyes, something you've definitely inherited from her is the DNA inside your mitochondria. These membrane-bound organelles are a vital part of aerobic respiration - how our cells use oxygen to transfer the energy from food into a form the body can use. Mitochondria are also involved in other metabolic and cellular processes, including ageing, cell signalling and cell death.
Thought to be descendants of bacteria that colonised our cells over 2 billion years ago, mitochondria contain their own genome. This was the first human genome to be sequenced, by Professor Fred Sanger and colleagues in 1981.
Ten years later, a revised version was released (with just 18 corrections to the 16 569-base-pair sequence). Some of these errors reflected rare mutations in the human placenta used as the source of DNA. Impressively, Sanger (retired by that point) could recall which freezer the original samples were stored in, allowing the researchers to resequence them.
Mutations in mitochondrial DNA (mtDNA) and/or nuclear DNA (in the genes containing the information to make parts of the mitochondria) can cause mitochondrial diseases. Common symptoms include muscle weakness, neurological problems, issues with sight and/or hearing, kidney disease, diabetes and heart disease.
While around one in 200 children is born with a potentially pathogenic mutation, only around one in 6500 develops severe mitochondrial disease, which can lead to death in early infancy. In the wider population, a UK study suggests that 9.2 in 100 000 people have recognisable mitochondrial disease caused by mutated mitochondrial DNA.
Highly dynamic organelles that fuse and split constantly, forming networks of varying size and dimensions. When healthy, they continuously supply chemical energy in the form of adenosine triphosphate (ATP) to all eukaryotic (nucleus-containing) cells. Mitochondria constitute 15 to 35 per cent of a cell's total mass.
The human form has 37 genes, 13 of which encode components of the cell's energy-transferring pathways. The effects of mutations in these genes are most evident in energy-hungry tissues such as the muscles, nerves and brain. Almost no recombination is thought to occur in the mitochondrial genome, but naturally occurring genetic variants are common. Evolutionary biologists can therefore use the strict maternal inheritance of mitochondrial DNA to track historical migration of populations across the world.
Similar to the cell's membrane, this is a fatty layer that contains a number of proteins. These include porins, which allow smaller molecules to cross the membrane freely.
Studded with the proteins of the electron transport chain, this is the site of the final stage of aerobic respiration. Also present is ATP synthase, the complex that catalyses the production of adenosine triphosphate (ATP).
In the final step of aerobic respiration, hydrogen ions (protons) cross from here to the matrix through the enzyme ATP synthase, creating adenosine triphosphate (ATP).
The folds of inner membrane that create compartments within mitochondria. Cristae increase the surface area available to house the complexes responsible for carrying out the reactions of aerobic respiration.
The innermost compartment of the mitochondrion, where several stages of aerobic respiration (including the Krebs cycle) take place. Contains enzymes, mitoribosomes and the mitochondrial DNA.
New centre for research
The Wellcome Trust has awarded £4.4 million to Newcastle University, which has contributed £1.4m itself, to establish the Wellcome Trust Centre for Mitochondrial Research. The researchers there - Professors Doug Turnbull, Robert Lightowlers, Patrick Chinnery, Mary Herbert, Zofia Chrzanowska-Lightowlers and Robert Taylor - will work to understand, treat and ultimately prevent mitochondrial diseases.
The Centre will advance current research that combines laboratory studies of basic aspects of mitochondrial biology with the expertise of clinical researchers who care for over 400 patients with mitochondrial diseases at clinics in Newcastle. One aspect of the work, preventing mitochondrial diseases, is already underway.
Turnbull and Herbert have been working on two methods to prevent transmission of the mutated mitochondrial DNA from mother to child. Both methods involve transferring the nuclear DNA (but not the mitochondria) from the mother's egg to a donor egg that has had its nuclear DNA removed but still contains mitochondria.
Other areas to be studied at the Centre include mitochondrial gene expression in health and disease (Robert Lightowlers and Zofia Chrzanowska-Lightowlers), the neurology of mitochondrial disease (Taylor) and the role of mitochondrial DNA in common complex disease (Chinnery).
This feature also appears in issue 70 of ‘Wellcome News’.
Image: Mitochondria (red) in the cell cytoplasm. Credit: Dr David Furness, Wellcome Images.