Next Generation Sequencing
18-24 July 2010
Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Course summary
In recent years, nucleic acid sequencing using next generation methods has emerged as a major transformative tool in the genomics field. This laboratory-based course is directed at scientists who will be generating and interpreting sequence information in their research and wish to gain a better understanding of the techniques involved and their applications. This course is not meant to replace the manufacturers’ training normally supplied with new instruments.
The course will consist of a mix of theoretical and practical elements focused on Solexa Sequencing on the Illumina platform. However, the course will familiarise participants with other technologies, allowing them to make informed decisions about which technology to apply to solve specific research questions they may face in the future.
Please note: For the data analysis components of the course, applicants will require a working knowledge of the Linux operating system.
Course programme
- Overview: Motivation and fundamental concepts
- Detailed description of Solexa sequencing technology
- Hands-on laboratory work: Amplifying DNA clusters and performing a sequencing run
- Lecture and practical work in analysis of data
- Data QC: How to determine if a run has performed well
- Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data
- IT, sample and project management, data storage and data sharing issues
- Methods for interpretation of the enormous amounts of sequence generated in a typical experiment
- Other technologies: Current and future.
- Applications of next generation sequencing: Cancer genomics, human variation analysis, etc.
Accompanying the lecture and practical sessions will be a series of seminars by invited speakers, who will highlight their ground-breaking work in applications of next generation sequencing. Confirmed speakers will be announced shortly.
Course instructors
Harold Swerdlow, Head of Sequencing Technology, The Wellcome Trust Sanger Institute, Cambridge, UK
Carol Churcher, Head of Sequencing Operations, The Wellcome Trust Sanger Institute, Cambridge, UK
Tony Cox, Head of Sequencing Informatics, The Wellcome Trust Sanger Institute, Cambridge, UK
How to apply
Target audience and prerequisites
Applicants should be postdoctoral scientists, senior PhD students or junior faculty members actively engaged in or soon to commence research involving next generation sequencing instrumentation. Applicants will require a working knowledge of the Linux operating system for the data analysis components of the course.
Cost
This is a residential course, without exception. Course fees are subsidised by the Wellcome Trust for non-commercial applicants but there is a charge of £750 towards board and lodging.The fee for commercial applicants is £3500.
Bursaries
Limited bursaries are available for academics (50 per cent of fee) and are awarded on merit. Please see our
bursaries page for application information and terms and conditions.
Applications
Applicants will be required to complete an application form containing a 300-word outline of the relevance of the course to their research. Please note that documentation supporting their application will be required from the applicant's supervisor/head of department.
To submit an application, please either:
- email for an application form to advancedcourses@hinxton.wellcome.ac.uk
- download the form and our terms and conditions.
Applications can be accepted by post or email. Sponsors' supporting letters must be returned, signed, by fax or post under separate cover. Please note: Incomplete applications will be rejected.
Applications and letters of support should be sent to:
Wellcome Trust Advanced Courses
Wellcome Trust Genome Campus
Hinxton
Cambridgeshire CB10 1SA
UK
F +44 (0) 1223 495130
E
advancedcourses@hinxton.wellcome.ac.uk
Deadlines
Now closed for applications