Technologies and Applications for Genome Analysis

18-27 April 2010

Wellcome Trust Genome Campus, Hinxton, Cambridge

Now closed for applications

Course summary

The course will provide a comprehensive overview and practical experience of the production and uses of DNA microarrays and related high-throughput methods for a broad range of applications in genome analysis.

Course programme

1. Expression analysis on whole genome oligonucleotide arrays
Students will extract and amplify RNA from a number of biological samples. Amplified material will be hybridised to whole genome expression arrays (one-colour platform) and the data extracted for gene expression profiling. Methods for RNA extraction, RNA amplification, assessment of RNA quality and considerations for single and two-colour assays will be discussed.In addition, the application of next generation sequencing for transcriptome analysis will be presented..

2. MicroRNA expression profiling
Students will prepare labelled material from RNA containing the microRNA fraction. This material will be hybridised to oligonucleotide arrays (two-colour platform) designed to interrogate all currently known vertebrate microRNAs.MicroRNA expression profiles will be generated for a number of biological samples.

3. Measuring genomic variation
Copy number variation in the genomes of test samples will be analysed using array comparative genomics.In addition, a range of applications for measuring genomic variation e.g. SNP genotyping will be covered.

4. Whole Genome DNA-protein Interactions
Chromatin immunoprecipitation of DNA-binding proteins will be performed using a mammalian cell line and the resultant material will be prepared for high-throughput Solexa sequencing. Genomic regions will be identified which interact with proteins including modified histones, chromatin remodelling complexes and transcription factors. Participants will also become familiar with similar approaches using genomic arrays in both practical and informatics sessions.

5. Informatics and data mining
The informatics necessary for the analysis of array and high-throughput sequencing data will be fully discussed in practical sessions. Data analysis will include data pre-processing (normalisation), inferential statistics, and descriptive (exploratory) statistics using public data and data generated on the course. Experimental design will also be discussed. The practical sessions will be complemented by short seminars related to the practicals as well as a series of invited seminars.

The practical sessions will be complemented by short seminars related to the practicals as well as a series of invited seminars.

Course instructors

  • Dr David Vetrie (University of Glasgow, UK)
  • Dr Cordelia Langford (Wellcome Trust Sanger institute, Cambridge)
  • Dr Peter Ellis (Wellcome Trust Sanger institute, Cambridge)
  • Dr Robert Andrews (Wellcome Trust Sanger Institute, Cambridge)

Guest speakers TBC shortly

How to apply

Prerequisites
Applicants should be postdoctoral scientists or senior PhD students actively engaged or soon to commence relevant research.

Cost
The course tuition fees are subsidised by the Wellcome Trust for scientists based in academic institutions anywhere in the world. This is a residential course, without exception, and there is a registration fee of £975 towards board and lodging for academic applicants. The fee for commercial (non-academic) applicants is £4000.

Bursaries
Limited bursaries are available for academics (50 per cent of fee) and are subject to open competition. Please see our bursaries page for application information and terms and conditions.

Applications
Applicants will be required to complete an application form containing a 300-word outline of the relevance of the course to their research. Please note that documentation supporting their application will be required from the applicant's supervisor/head of department.

To submit an application, please either:

Applications can be accepted by post or email. Sponsors' supporting letters must be returned, signed, by fax or post under separate cover. Please note: Incomplete applications will be rejected.

Applications and letters of support should be sent to:

Wellcome Trust Advanced Courses
Wellcome Trust Genome Campus
Hinxton
Cambridgeshire CB10 1SA
UK

F +44 (0)1223 495130
E advancedcourses@hinxton.wellcome.ac.uk

Deadlines
Now closed for applications

Home  >  Education resources  >  Courses and conferences  >  Advanced Courses  >  Courses  > Microarrays: Methods and applications for genome analysis