Protein Interactions and Networks

8-16 August 2013

Wellcome Trust Genome Campus , Hinxton, Cambridge

Now closed for applications. Please email to join our mailing list.

Course summary

This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.

For 2013, the course has been expanded to cover protein interactions by FRET microscopy (lead instructor: Professor Fred S. Wouters, University of Göttingen, Germany) and will again combine practical work with computer-based data analysis.

The practical programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.

Programme

Protein Complexes by Mass Spectrometry

affinity purification using TAP tag approach
protein identification by Mass Spectrometry.

Yeast Two-Hybrid

rapid methods for making Bait and Prey clones
using mating assays to investigate specific interactions
library screening: faster ways to find new partners.

Binary Protein Interactions/BIAcore

low affinity extracellular receptor-ligand interactions by AVEXIS
biophysical protein interaction parameters (binding constants and kinetics).

Bioinformatics

network and pathway visualisation
protein interaction prediction and analysis
data integration.

Protein Interactions by FRET microscopy

sample preparation
intensity-based FRET microscopy
Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
image/data analysis.

Course instructors

Jyoti Choudhary (Wellcome Trust Sanger Institute, Hinxton, UK)
Chris Sanderson (University of Liverpool, UK)
Gavin Wright (Wellcome Trust Sanger Institute, Hinxton, UK)
Anton Enright (European Bioinformatics Institute, Hinxton, UK)
Fred S. Wouters (University of Göttingen, Germany)

Guest speakers to include

Woj Wojtowicz (University of California, Berkeley, USA)
Claus Jørgensen (The Institute of Cancer Research, UK)

Feedback from previous courses

“This course was very well organized, furthermore the guest speakers' talks were fantastic. Everything we learned were very useful and I am very excited to apply what I have learned in our lab/projects.”

“Fantastic course! I really enjoyed it and got a lot out of it.”

How to apply

Prerequisites
Applicants should be postdoctoral scientists or PhD students engaged in or soon to commence relevant research.

Cost
The course tuition fees are subsidised by The Wellcome Trust for scientists based in non-commercial institutions anywhere in the world. This is a residential course, without exception, and there is a fee of £950 towards board and lodging for non-commercial applicants. The fee for commercial applicants is £3600.

Bursaries
Limited bursaries are available for non-commercial applicants (50 per cent of fee) and are subject to open competition. If you would like to apply for a bursary, please complete the bursary section of the online application form (see below for application process).

Applications
Now closed for applications. Please email to join our mailing list.