Next Generation Sequencing
6-13 April 2014
Wellcome Trust Genome Campus, Hinxton, Cambridge
Now closed for applications. Please
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Next-generation sequencing has become the premier tool in genetic and genomic analysis. This laboratory-based course is directed at scientists who will be generating and interpreting sequence information in their research and wish to gain a better understanding of the techniques involved and their applications. This course is not meant to replace the manufacturers’ training normally supplied with new instruments, nor is it intended to be a training course for those solely interested in next-generation sequencing bioinformatics.
The course will include theoretical and practical information on all of the next-generation sequencing systems available and those on the near horizon. The laboratory element will cover a mix of technologies that will be determined nearer the time, but will be chosen from platforms like the Ion Torrent, PacBio and Illumina Miseq / HiSeq. One aim of the course is to allow participants to make informed decisions about which technology to apply to solve specific research questions they may face in the future. A variety of applications will be covered as time permits, e.g., RNA-Seq, target enrichment, bacterial sequencing, cancer genomics, human variation analysis, etc. All the basic techniques of post-sequencing analysis will be covered, QC, alignment, assembly, variant calling, etc.
Please note: For the data analysis components of the course, applicants will require a working knowledge of the Linux operating system. This can be obtained by various methods and will be enforced.
• Making libraries from samples
• Both theory and practical sessions
• Overview: Motivation and fundamental concepts
• Detailed description of prominent sequencing technologies: current and future (3rd generation) platforms
• Hands-on laboratory work: preparing chips for sequencing and performing sequencing runs on various platforms
• Data QC: How to determine if a run has performed well
• Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data
• IT and the data storage problem
Accompanying the lecture and practical sessions will be a series of seminars by invited speakers, who will highlight their ground-breaking work in applications of next generation sequencing (cancer, human diseases, pathogens, etc).
Harold Swerdlow (Head of Sequencing Technology, The Wellcome Trust Sanger Institute, Cambridge, UK)
Tony Cox (Head of Sequencing Informatics, The Wellcome Trust Sanger Institute, Cambridge, UK)
2012 guest speakers (2014 speakers TBA shortly)
Dominic Kwiatkowski (Wellcome Trust Sanger Institute, UK)
Richard Durbin (Wellcome Trust Sanger Institute, UK)
Jenny Ekholm (Pacific Biosciences)
Peter Campbell (Wellcome Trust Sanger Institute, UK)
Manfred Lee (Life Technologies)
Feedback from the 2012 course
“The instructors were excellent, professional and very helpful. Thanks again for a most enjoyable course.”
“A big thank you to the course instructors and the lab demonstrators. All of them were helpful, enthusiastic and knowledgeable - without exception!”
“The course was extremely satisfying. The attention of all instructors, especially the organizers, was very generous. The knowledge and skills acquired will certainly be valuable for my work.”
How to apply
Target audience and prerequisites
Applicants should be postdoctoral scientists, senior PhD students or junior faculty members actively engaged in or soon to commence research involving next generation sequencing instrumentation.
This course includes a substantial element of sequence data QC and analysis performed on Linux-based computers. To benefit fully from the course it is essential that successful applicants are comfortable working in a Linux-based environment prior to the course. In particular, applicants will be expected to use the command line terminal interface.
Applicants will need to be familiar with, and able to perform, the following:
creating, copying, moving and deleting text files and directories, modifying file and directory permissions, navigating directory structures, examining the contents of text files, executing programs from the command line using pipes and output redirection, compressing and decompressing files (using zip or gzip)
The course tuition fees are subsidised by the Wellcome Trust for scientists based in non-commercial institutions anywhere in the world. This is a residential course and there is a registration fee of £950 towards board and lodging for non-commercial applicants. The fee for commercial applicants is £4000.
Limited bursaries are available for non-commercial applicants (50 per cent of fee) and are subject to open competition. If you would like to apply for a bursary, please complete the bursary section of the online application form (see below for application process).
Now closed for applications. Please email to join our mailing list.