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Exome Sequencing

11-19 September 2014

Wellcome Trust Genome Campus, Hinxton, Cambridge

Application deadline: 30 May 2014

Course summary

This popular laboratory- and computer-based training course provides a comprehensive overview of the latest principles and techniques for exome sequencing, also known as pulldown, sequence capture and targeted sequencing.
Participants will gain practical laboratory experience as well as training in downstream data analysis, interpretation and validation.

Solution hybrid capture is used to enrich for all known exon sequences (the exome) or for customised regions from genomic DNA, for the purpose of sequencing the regions of interest. The enriched targets are sequenced using next generation technology to a high coverage and analysed for variants. For comparison, the concepts for array CGH (aCGH) will be covered as an approach for analysing somatic DNA copy number variation

With increased use of analytical methods using DNA isolated from formalin fixed clinical samples, attention will be paid to the consequences of sample processing, including duration of exposure to formalin fixation.

Programme

Material to be covered will include:

  • Sample quality assessment and logistics
  • DNA fragmentation and quantification
  • DNA library preparation
  • Sequence capture
  • Bait design
  • Overview of Illumina sequencing
  • Sequence mapping
  • QC metrics
  • SNP/insertion/deletion calling
  • SNP consequence analysis
  • Protein impact analysis
  • Variant annotation
  • Case-only analysis
  • Case/control analysis
  • Rare SNP discovery
  • Impact of formalin fixation on data quality ofexome seqeuncing and CGH analysis

Teaching will be complemented by opportunities to interact with academic and technical experts working at the cutting edge of the field.

Course instructors

Dr Cordelia Langford (Wellcome Trust Sanger Institute, Hinxton, UK)
Dr Peter Ellis (Wellcome Trust Sanger Institute, Hinxton, UK)
Dr Robert Andrews (Cardiff University, UK)
Dr Matthew Breen (North Carolina State University, USA)
Dr Audrey Hendricks (Wellcome Trust Sanger Institute, Hinxton, UK)

How to apply

Prerequisites
Applicants should be post-doctoral scientists or third-year PhD students undertaking their own research, core-lab service providers are also encouraged to apply. Applicants must be familiar with standard molecular biology lab techniques. Basic UNIX skills and the ability to confidently use Excel are also required.

Cost
The course tuition fees are subsidised by The Wellcome Trust for scientists based in non-commercial institutions anywhere in the world. This is a residential course and there is a fee of £995 towards board and lodging for non-commercial applicants. The fee for commercial applicants is £4000.

Bursaries
Limited bursaries are available for non-commercial applicants (up to 50 per cent of fee) and are subject to open competition. If you would like to apply for a bursary, please complete the bursary section of the online application form (see below for application process).

Applications
Application forms for this course can now be completed online. If you have any problems with the online application process, please email us.

Please note: Applications must be supported by a recommendation from a scientific sponsor. This can be your supervisor or head of department. A request for a supporting statement will be sent to your nominated sponsor automatically during the application process. Applicants must ensure that their sponsor provides this supporting statement by the application deadline.

Deadlines
Application deadline: 30 May 2014

Wellcome Trust, Gibbs Building, 215 Euston Road, London NW1 2BE, UK T:+44 (0)20 7611 8888