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Protein Interactions and Networks

4-12 August 2014

Wellcome Trust Genome Campus, Hinxton, Cambridge

Deadline for applications: 25 April 2014

Course summary

This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.

The practical programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.

Programme

Protein Complexes by Mass Spectrometry

  • affinity purification using TAP tag approach
  • protein identification by Mass Spectrometry.

Yeast Two-Hybrid

  • rapid methods for making Bait and Prey clones
  • using mating assays to investigate specific interactions
  • library screening: faster ways to find new partners.

Binary Protein Interactions/BIAcore

  • low affinity extracellular receptor-ligand interactions by AVEXIS
  • biophysical protein interaction parameters (binding constants and kinetics).

Bioinformatics

  • network and pathway visualisation
  • protein interaction prediction and analysis
  • data integration.

Protein Interactions by FRET microscopy

  • sample preparation
  • intensity-based FRET microscopy
  • Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
  • image/data analysis.

Course instructors

Jyoti Choudhary (Wellcome Trust Sanger Institute, Hinxton, UK)
Chris Sanderson (University of Liverpool, UK)
Gavin Wright (Wellcome Trust Sanger Institute, Hinxton, UK)
Anton Enright (European Bioinformatics Institute, Hinxton, UK)
Fred S. Wouters (University of Göttingen, Germany)

2013 Guest speakers (2014 speakers TBA shortly)

Woj Wojtowicz (University of California, Berkeley, USA)
Claus Jørgensen (The Institute of Cancer Research, UK)

Feedback from previous courses

“This course was very well organized, furthermore the guest speakers' talks were fantastic. Everything we learned were very useful and I am very excited to apply what I have learned in our lab/projects.”

“Fantastic course! I really enjoyed it and got a lot out of it.”

“I feel that this was a brilliantly designed course, we had some great instructors, each one was so good in their work and always ready to help.”

How to apply

Prerequisites
Applicants should be postdoctoral scientists or PhD students engaged in or soon to commence relevant research.

Cost
The course tuition fees are subsidised by The Wellcome Trust for scientists based in non-commercial institutions anywhere in the world. This is a residential course and there is a fee of £950 towards board and lodging for non-commercial applicants. The fee for commercial applicants is £3600.

Bursaries
Limited bursaries are available for non-commercial applicants (up to 50 per cent of fee) and are subject to open competition. If you would like to apply for a bursary, please complete the bursary section of the online application form (see below for application process).

Applications
Application forms for this course can now be completed online. If you have any problems with the online application process, please email us.

Please note: Applications must be supported by a recommendation from a scientific sponsor. This can be your supervisor or head of department. A request for a supporting statement will be sent to your nominated sponsor automatically during the application process. Applicants must ensure that their sponsor provides this supporting statement by the application deadline

Deadlines
Deadline for applications: 25 April 2014

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