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Protein Interactions and Networks

3-11 August 2015

Wellcome Trust Genome Campus, Hinxton, Cambridge

Now closed for applications, please email to join our mailing list.

Course summary

This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.

The practical programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.


Protein Complexes by Mass Spectrometry

  • affinity purification using TAP tag approach
  • protein identification by Mass Spectrometry.

Yeast Two-Hybrid

  • rapid methods for making Bait and Prey clones
  • using mating assays to investigate specific interactions
  • library screening: faster ways to find new partners.

Binary Protein Interactions/BIAcore

  • low affinity extracellular receptor-ligand interactions by AVEXIS
  • biophysical protein interaction parameters (binding constants and kinetics).


  • network and pathway visualisation
  • protein interaction prediction and analysis
  • data integration.

Protein Interactions by FRET microscopy

  • sample preparation
  • intensity-based FRET microscopy
  • Fluorescence Lifetime Imaging (FLIM)-based FRET microscopy
  • image/data analysis.

Course instructors

Jyoti Choudhary (Wellcome Trust Sanger Institute, Hinxton, UK)
Chris Sanderson (University of Liverpool, UK)
Gavin Wright (Wellcome Trust Sanger Institute, Hinxton, UK)
Anton Enright (European Bioinformatics Institute, Hinxton, UK)
Fred S. Wouters (University of Göttingen, Germany)

Confirmed guest speakers

Edward Huttlin (Harvard Medical School, USA)
Engin Özkan (University of Chicago, USA)

Feedback from the 2014 course

“This course was very well organized, furthermore the guest speakers' talks were fantastic. Everything we learned were very useful and I am very excited to apply what I have learned in our lab/projects.”

“Fantastic course! I really enjoyed it and got a lot out of it.”

“I feel that this was a brilliantly designed course, we had some great instructors, each one was so good in their work and always ready to help.”

How to apply

Applicants should be postdoctoral scientists or PhD students engaged in or soon to commence relevant research.

The course tuition fees are subsidised by The Wellcome Trust for scientists based in non-commercial institutions anywhere in the world. This is a residential course and there is a fee of £1000 towards board and lodging for non-commercial applicants. The fee for commercial applicants is £3780.

Limited bursaries are available for non-commercial applicants (up to 50 per cent of fee) and are subject to open competition. If you would like to apply for a bursary, please complete the bursary section of the online application form (see below for application process).

Now closed for applications, please email to join our mailing list.

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